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- Title
Expression of the sweet-tasting plant protein brazzein in Escherichia coli and Lactococcus lactis: a path toward sweet lactic acid bacteria.
- Authors
Berlec, Aleš; Jevnikar, Zala; Majhenič, Andreja Čanžek; Rogelj, Irena; Štrukelj, Borut
- Abstract
Brazzein is an intensely sweet-tasting plant protein with good stability, which makes it an attractive alternative to sucrose. A brazzein gene has been designed, synthesized, and expressed in Escherichia coli at 30 °C to yield brazzein in a soluble form and in considerable quantity. Antibodies have been produced using brazzein fused to His-tag. Brazzein without the tag was sweet and resembled closely the taste of its native counterpart. The brazzein gene was also expressed in Lactococcus lactis, using a nisin-controlled expression system, to produce sweet-tasting lactic acid bacteria. The low level of expression was detected with anti-brazzein antibodies. Secretion of brazzein into the medium has not led to significant yield increase. Surprisingly, optimizing the codon usage for Lactococcus lactis led to a decrease in the yield of brazzein.
- Subjects
PLANT proteins; GENE expression; PLANT gene mapping; ESCHERICHIA coli; LACTOCOCCUS lactis; NISIN; LACTIC acid bacteria genetics
- Publication
Applied Microbiology & Biotechnology, 2006, Vol 73, Issue 1, p158
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-006-0438-y