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- Title
IN VITRO MEDIUM TERM CONSERVATION OF SWEET POTATO GENOTYPES USING MANNITOL AND SORBITOL.
- Authors
Cioloca, Mihaela; Tican, Andreea; Bădărău, Carmen; Bărăscu, Nina; Popa, Monica
- Abstract
The study is aimed at establishing an efficient protocol for "in vitro" medium term conservation of sweet potato. Three medium variants and three different temperatures were used for this purpose. In this experiment 6 varieties of sweet potato were tested (Juhwangmi, KSC1, Yulmi, KSP1, Hayanmi and VSP1). Uninodal segments of fully developed sweet potato plants already established "in vitro" were cultured on MS basal medium with and without osmotic regulators (mannitol and sorbitol). The cultures were incubated at three different temperatures: 24±2ºC, 20±2ºC and 16±2ºC. After 140 days of "in vitro" conservation shoots height, number of leaves, number of roots and survival rate (%) were measured. Culture condition variants V1 (MS, 24±2ºC) and V2 (MS + 10g/l mannitol + 10 g/l sorbitol, 20±2ºC) ensured the best results regarding shoots height, number of leaves and number of roots. The highest survival rate (87.78% and 84.44% respectively) were observed in the V2 and V3 (MS + 15g/l mannitol + 15 g/l sorbitol, 16±2ºC) culture condition variants. The slow growth technique allowed the successful "in vitro" medium term conservation of sweet potato genotypes.
- Subjects
SWEET potatoes; MANNITOL; SORBITOL; POTATOES; GENOTYPES
- Publication
Romanian Agricultural Research, 2021, Issue 38, p1
- ISSN
1222-4227
- Publication type
Article