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- Title
Expression of advanced glycation end products and related molecules in diabetic fibrovascular epiretinal membranes.
- Authors
Abu El-Asrar, Ahmed M.; Missotten, Luc; Geboes, Karel
- Abstract
Purpose: To investigate associations between expressions of advanced glycation end products (AGEs), transforming growth factor-β (TGF-β), tumour necrosis factor-α (TNF-α) and integrins and correlations between their expression and level of vascularization and proliferative activity in diabetic fibrovascular epiretinal membranes. Methods: Membranes from eight patients with active proliferative diabetic retinopathy and nine patients with inactive proliferative diabetic retinopathy were studied by immunohistochemistry. Results: Blood vessels expressed AGEs, TGF-β, TNF-α and αvβ3 integrin in 5, 13, 8 and 8 membranes, respectively. Stromal cells expressed AGEs, TNF-α and αvβ3 integrin in 15, 13 and 3 membranes, respectively. There was no immunoreactivity for αvβ5, α5β1 and α2β1 integrins. There were significant correlations between number of blood vessels expressing CD34 and number of blood vessels expressing AGEs ( rs = 0.496; P = 0.043), TGF-β ( rs = 0.777; P < 0.001) and TNF-α ( rs = 0.699; P = 0.002). There were significant correlations between number of blood vessels expressing AGEs and number of blood vessels expressing TGF-β ( rs = 0.532; P = 0.028) and TNF-α ( rs = 0.626; P = 0.007). The correlation between number of blood vessels expressing TNF-α and αvβ3 integrin was significant ( rs = 0.617; P = 0.008). Number of blood vessels expressing CD34 ( P = 0.001), TGF-β ( P = 0.006) and TNF-α ( P = 0.002) and stromal cells expressing AGEs ( P = 0.001) and TNF-α ( P = 0.004) were significantly higher in active membranes than in inactive membranes. Conclusion: Interactions of AGEs, TGF-β, TNF-α and αvβ3 integrin might be involved in pathogenesis of proliferative diabetic retinopathy fibrovascular proliferation.
- Subjects
TUMOR necrosis factors; BIOLOGICAL membranes; BLOOD vessels; IMMUNOHISTOCHEMISTRY; INTEGRINS
- Publication
Clinical & Experimental Ophthalmology, 2010, Vol 38, Issue 1, p57
- ISSN
1442-6404
- Publication type
Article
- DOI
10.1111/j.1442-9071.2010.02194.x