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- Title
SNP GENOTYPING BY TAQMAN ALLELE DISCRIMINATION TECHNIQUE.
- Authors
NEGURĂ, LUCIAN; MIRON, LUCIAN; MARINCA, MIHAI; AZOICĂI, DOINA; NEGURĂ, ANCA
- Abstract
Breast cancer is the most frequent neoplasm in women worldwide and the principal cause of deaths by cancer, the majority being by metastatic disease. About half of breast tumors are hormone dependent, and in post- menopause women the preferred first line treatment uses third generation aromatase inhibitors. Aromatase is encoded by CYP19 gene on 15q21.1, and there is strong evidence that mutations in this gene affect its expression, with direct consequences on cancer phenotype and response to treatment. Several single nucleotide polymorphisms have been studied on CYP19A1 transcription variant, notably rs727479, rs10046, rs4646 and rs700518. We implemented a Taqman-based allele discrimination assay for the rapid investigation of the 4 SNPs in CYP19A1. We genotyped 22 metastatic breast cancer patients by the technique described.
- Subjects
BREAST cancer; AROMATASE; SINGLE nucleotide polymorphisms
- Publication
Analele Stiintifice ale Universitatii Alexandru Ioan Cuza din Iasi. Sectiunea II A, Genetica si Biologie Moleculara, 2015, Vol 16, Issue 2, p71
- ISSN
1582-3571
- Publication type
Article