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- Title
Intestinal stroma guides monocyte differentiation to macrophages through GM-CSF.
- Authors
Kvedaraite, Egle; Lourda, Magda; Mouratidou, Natalia; Düking, Tim; Padhi, Avinash; Moll, Kirsten; Czarnewski, Paulo; Sinha, Indranil; Xagoraris, Ioanna; Kokkinou, Efthymia; Damdimopoulos, Anastasios; Weigel, Whitney; Hartwig, Olga; Santos, Telma E.; Soini, Tea; Van Acker, Aline; Rahkonen, Nelly; Flodström Tullberg, Malin; Ringqvist, Emma; Buggert, Marcus
- Abstract
Stromal cells support epithelial cell and immune cell homeostasis and play an important role in inflammatory bowel disease (IBD) pathogenesis. Here, we quantify the stromal response to inflammation in pediatric IBD and reveal subset-specific inflammatory responses across colon segments and intestinal layers. Using data from a murine dynamic gut injury model and human ex vivo transcriptomic, protein and spatial analyses, we report that PDGFRA+CD142−/low fibroblasts and monocytes/macrophages co-localize in the intestine. In primary human fibroblast-monocyte co-cultures, intestinal PDGFRA+CD142−/low fibroblasts foster monocyte transition to CCR2+CD206+ macrophages through granulocyte-macrophage colony-stimulating factor (GM-CSF). Monocyte-derived CCR2+CD206+ cells from co-cultures have a phenotype similar to intestinal CCR2+CD206+ macrophages from newly diagnosed pediatric IBD patients, with high levels of PD-L1 and low levels of GM-CSF receptor. The study describes subset-specific changes in stromal responses to inflammation and suggests that the intestinal stroma guides intestinal macrophage differentiation.Stromal cells are key players in immune cell homeostasis. Here, the authors decipher subset-specific human stromal responses in inflammatory bowel disease and suggest that intestinal PDGFRA+CD142−/low fibroblasts guide monocyte transition to macrophages in human gut through GM-CSF.
- Publication
Nature Communications, 2024, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-024-46076-3