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- Title
Manganese(II)-doped carbon dots as effective oxidase mimics for sensitive colorimetric determination of ascorbic acid.
- Authors
Zhuo, Shujuan; Fang, Jing; Li, Meng; Wang, Jing; Zhu, Changqing; Du, Jinyan
- Abstract
A colorimetric assay is presented for the determination of ascorbic acid (AA). Manganese(II) doped carbon dots (Mn-CDs) were prepared by a convenient hydrothermal route and are shown to possess oxidase-like catalytic ability. They catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by dissolved oxygen to form a blue colored product (oxTMB). AA can reduce blue oxTMB to colorless TMB. The fading of the blue color (measured at 652 nm) can be applied for quantifying AA in the 50 to 2500 nM concentration range and with a 9 nM detection limit. The method was successfully used for the quantitation of AA in real samples.
- Subjects
VITAMIN C; DETECTION limit; CARBON; MANGANESE; DOPING agents (Chemistry); SERUM; OXIDASES; OPTICAL sensors
- Publication
Microchimica Acta, 2019, Vol 186, Issue 12, p1
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-019-3887-6