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- Title
Direct electrochemistry and electrocatalysis of hemoglobin in a multilayer {nanogold/PDDA}<sub>n</sub> inorganic–organic hybrid film.
- Authors
Dianyong Tang; Biyun Xia; Yuanqin Zhang
- Abstract
A new amperometric biosensor for hydrogen peroxide (H2O2) has been developed that is based on direct electrochemistry and electrocatalysis of hemoglobin (Hb) in a multilayer inorganic–organic hybrid film. o-Phenylenediamine (PDA) was electropolymerized onto a glassy carbon electrode (GCE), and then negatively charged nanogold particles and positively charged poly(diallyldimethylammonium chloride) (PDDA) were alternately assembled on the PDA/GCE surface. Finally, Hb was electrostatically adsorbed on the surface of gold nanoparticles. The electrochemical behavior of the resulting biosensor (Hb/{nanogold/PDDA}n/PDA/GCE) was assessed and optimized. The performance and factors influencing the biosensor were studied in detail. Under optimal conditions, the immobilized Hb displayed good electrocatalytic response to the H2O2 reduction ranging from 1.3 µM to 1.4 mM with a detection limit of 0.8 µM (at 3δ). In addition, the biosensor exhibited rapid response, good reproducibility, and long-term stability.
- Subjects
HEMOGLOBINS; ELECTROCHEMISTRY; BIOSENSORS; ELECTROCATALYSIS; SEMICONDUCTOR films; AMINES
- Publication
Microchimica Acta, 2008, Vol 160, Issue 3, p367
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-007-0840-x