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- Title
Differential effects between cyclooxygenase-2 inhibitors and siRNA on vascular endothelial growth factor production in head and neck squamous cell carcinoma cell lines.
- Authors
Park, Seok-Woo; Kim, Hyo-Sun; Hah, Jeong-Hun; Kim, Kwang-Hyun; Heo, Dae-Seog; Sung, Myung-Whun
- Abstract
Background: Several researchers have observed that cyclooxygenase-2 (COX-2) inhibitors display anticancer effects only at higher concentrations than doses that block COX-2 activity in head and neck squamous cell carcinoma (HNSCC) cells. Methods: To better understand the exact anticancer mechanism of COX-2-inhibitors, we compared the effects of pharmacologic inhibitors to those of small-interfering RNA against COX-2 on cell-growth, vascular endothelial growth factor (VEGF) production, and intracellular signaling in HNSCC cell lines. Results: We observed in HNSCC cells, that COX-2-siRNA induced an inhibitory effect on intracellular signaling, but unlike the pharmacologic inhibitors, did not affect cell proliferation. Whereas the chemical inhibitors increased VEGF synthesis even at low doses, COX-2-siRNA showed differential inhibition of VEGF production according to expression patterns of COX-1 and COX-2 in tested cells. Conclusion: The majority of the anticancer effects of COX-2-inhibitors in HNSCC cells seem to result from COX-2-independent action, suggesting that COX-1 and COX-2 may contribute to VEGF synthesis in cancer cells through a prostaglandin-dependent mechanism. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
- Subjects
CELL differentiation; CYCLOOXYGENASE 2 inhibitors; VASCULAR endothelial growth factors; SQUAMOUS cell carcinoma; CANCER treatment; CELL lines
- Publication
Head & Neck, 2010, Vol 32, Issue 11, p1534
- ISSN
1043-3074
- Publication type
Article
- DOI
10.1002/hed.21362