We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Purification of Bacillus licheniformis Lipase and its Application as an Additive in Detergent for Destaining.
- Authors
Bredai, Rania; Ben Romdhane, Ines Belhaj; Bouchaala, Imen; Srih Belghith, Karima; Belghith, Hafedh
- Abstract
In this study, we aimed to optimize the nutritional and environmental conditions for the production of a novel lipase (LBL) from Bacillus licheniformis (GenBank accession no. MT118724). This strain was characterized by morphological and biochemical assays and Sanger sequencing of 16S rDNA. The crude lipolytic activity reached a maximum level 7.5 U mL-1 at 40 °C and pH 8.0 using olive oil as substrate. Additionally, the crude enzyme maintained 100% of its initial activity after incubation for 1 h at 50 °C and pH 9.0. It is mandatory to note that LBL lipase displayed appreciable stability over a wide pH range and extreme temperatures. After purification, the optimal lipolytic activity was observed at pH 8.0 and 40 °C. LBL was shown to be a monomeric protein with an estimated molecular weight of 40 kDa. This novel lipase exhibited high stability and excellent compatibility compared to lipase extracted from Thermomyces lanuginosa (Lipolase® from Novozymes, Denmark) toward various detergents. Washing performance analysis revealed that it efficiently removes tomato sauce stain from cotton cloth. All these interesting enzymatic properties favor this new lipase as a potent candidate for applications in detergent formulations.
- Subjects
DENMARK; BACILLUS licheniformis; LIPASES; DETERGENTS; MOLECULAR weights; OLIVE oil; TOMATO sauces
- Publication
Journal of Surfactants & Detergents, 2021, Vol 24, Issue 5, p835
- ISSN
1097-3958
- Publication type
Article
- DOI
10.1002/jsde.12514