We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Epidermal melanocytes metabolize extracellular nucleotides by purinergic enzymes.
- Authors
Naasani, Liliana Ivet Sous; Azevedo, Jéssica Gonçalves; Sévigny, Jean; Franco de Oliveira, Tiago; Maria-Engler, Silvya Stuchi; Wink, Márcia Rosângela
- Abstract
The human epidermal melanocyte (hEM) are melanin-producing cells that provide skin pigmentation and protection against ultraviolet radiation. Although purinergic signaling is involved in skin biology and pathology, the presence of NTPDase members, as well as the rate of nucleotides degradation by melanocytes were not described yet. Therefore, in this study, we analyzed the expression of ectonucleotidases in hEM derived from discarded foreskin of male patients. The expression of purinergic enzymes was confirmed by mRNA and flow cytometry. Among the ectonucleotidases, ectonucleoside triphosphate diphosphohydrolase1 (NTPDase1) and ecto-5´-nucleotidase were the ectoenzymes with higher expressions. The hydrolysis rate for ATP, ADP, and AMP was low in comparison to other primary cells already investigated. The amount of ATP in the culture medium was increased after a scratch wound and decreased to basal levels in 48 h, while the NTPDase1 and P2X7 expressions increased. Therefore, it is possible to suggest that after cell injury, the ATP released by hEM into the extracellular space will be hydrolyzed by ectonucleotidases as the NTPDase1 that will control the levels of nucleotides in the skin micro-environment. Schematic representation of ectonucleotidases expression in human epidermal melanocyte cells. Melanocytes expressed mRNA for ENTPD1, ENTPD2, ENTPD3, ENTPD5, ENTPD6, CD73, ENPP1, ENPP2 and ENPP3, being the ENTPD2 expressed at lower levels. The expression of these ectonucleotidases at protein level was confirmed by flow cytometry that showed the ENTPD1 and CD73 expressed at higher levels on the cell surface.
- Subjects
PURINERGIC receptors; MELANOCYTES; GENE expression; NUCLEOTIDES; EXTRACELLULAR space; EXTRACELLULAR enzymes
- Publication
Biochemistry & Cell Biology, 2023, Vol 101, Issue 3, p259
- ISSN
0829-8211
- Publication type
Article
- DOI
10.1139/bcb-2022-0058