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- Title
17β-Estradiol Up-Regulates Prostacyclin Production in Cultured Human Uterine Myometrial Cells Via Augmentation of Both Cyclooxygenase-1 and Prostacyclin Synthase Expression.
- Authors
Korita, Daizo; Itoh, Hiroaki; Sagawa, Norimasa; Yura, Shigeo; Yoshida, Masahiro; Kakui, Kazuyo; Takemura, Maki; Fujii, Shingo
- Abstract
Objective:To investigate whether 17β-estradiol elevates prostacyclin (PGI2) production in human myometrial cells in the middle of gestation.Methods:The concentration of 6-keto-PGF1α, a stable metabolite of PGI2, in the culture medium was assessed using enzyme-linked immunosorbent assay (ELISA). Western blot analysis and quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) using TaqMan (Applied Biosystems, Foster City, CA) technology were performed to evaluate the expression of cytosolic phospholipase A2 (cPLA2), cyclooxygenase-1 (COX-1), COX-2, and prostacyclin synthase (PGIS) in cultured human myometrial cells prepared from second trimester pregnant women (n = 3) after stimulation with 17β-estradiol.Results:Treatment with 17β-estradiol (4-400 nM) dose-dependently elevated PGI2 secretion from cultured human myometrial cells. Western blot analysis detected cPLA2 and COX-1 and PGIS protein expression in the cultured human myometrial cells; however, COX-2 protein expression was below the detection sensitivity. Stimulation with 40-nM 17β-estradiol significantly up-regulated protein and mRNA expression of both COX-1 and PGIS.Conclusion:17βEstradiol from placenta may contribute to the augmentation of PGI2 production in the human myometrium in the middle of gestation via up-regulation of both COX-1 and PGIS expression.
- Publication
Reproductive Sciences, 2004, Vol 11, Issue 7, p457
- ISSN
1933-7191
- Publication type
Article
- DOI
10.1016/j.jsgi.2004.04.002