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- Title
Xuhuai Goat H-FABP Gene Clone, Subcellular Localization of Expression Products and the Preparation of Transgenic Mice.
- Authors
Yin, Yan-hui; Li, Bi-chun; Wei, Guang-hui; Zhu, Cai-ye; Li, Wei; Zhang, Ya-ni; Du, Li-xin; Cao, Wen-guang
- Abstract
The aim of this study was to clone the heart-type fatty acid binding protein ( H-FABP) gene of Xuhuai goat, to explore it bioinformatically, and analyze the subcellular localization using enhanced green fluorescent protein ( EGFP). The results showed that the coding sequence (CDS) length of Xuhuai goat H-FABP gene was 402 bp, encoding 133 amino acids (GenBank accession number AY466498.1). The H-FABP cDNA coding sequence was compared with the corresponding region of human, chicken, brown rat, cow, wild boar, donkey, and zebrafish. The similarity were 89%, 76%, 85%, 84%, 93%, 91%, 70%, respectively. For the corresponding amino acid sequences, the similarity were 90%, 79%, 88%, 97%, 95%, 94%, 72%, respectively. This study did not find the signal peptide region in the H-FABP protein; it revealed that H-FABP protein might be a nonsecreted protein. H-FABP expression was detected in vitro by reverse transcription-polymerase chain reaction (RT-PCR), and the EGFP-H-FABP fusion protein was localized to the cytoplasm. The gene could also be transiently and permanently expressed in mice.
- Subjects
CLONING; TRANSGENIC mice; DNA polymerases; PROTEIN analysis; AMINO acid sequence
- Publication
DNA & Cell Biology, 2012, Vol 31, Issue 5, p753
- ISSN
1044-5498
- Publication type
Article
- DOI
10.1089/dna.2011.1449