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- Title
Investigation of Polymorphic Variants of PPARD and APOE Genes in Turkish Coronary Heart Disease Patients.
- Authors
Yılmaz-Aydogan, Hulya; Kucukhuseyin, Ozlem; Kurnaz, Ozlem; Akadam-Teker, Basak; Kurt, Ozlem; Tekeli, Atike; Ozturk, Oguz; Isbir, Turgay
- Abstract
The aim of this study was to determine the role of polymorphic variants of apolipoprotein E ( APOE) and peroxisome proliferator-activated receptor delta ( PPARD) genes in the development of coronary heart disease (CHD), and the PPARD and APOE gene-gene interaction in a Turkish population. This study was carried out using a sample of 223 patients with CHD (103 with diabetes and 120 without diabetes) and 101 controls. PPARD +294T/C and APOE genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism technique. The PPARD and APOE genotype distributions were the same between study groups ( p>0.05). In the nondiabetic CHD patients, the PPARD +294 C allele showed higher serum low-density lipoprotein cholesterol (LDL-C) level than the common +294 TT homozygote genotype (3.83±1.01 vs. 3.33±1.14, p=0.015). In addition, a significant association between APOE 4 and PPARD +294 C alleles was detected based on their effects on LDL-C in the nondiabetic CHD patients (+294 C/ APOE4: 4.43±0.88 vs. +294 TT/non APOE 4: 3.48±1.09, p=0.009). This association indicated the interaction of two genes on plasma LDL-C levels ascended in the order +294 T<+294 T- APOE 4<+294 C< APOE 4<+294 C- APOE 4. The PPARD +294 C allele was associated with higher incidence of left ventricular hypertrophy (LVH) in all male patients with body mass index >27. In addition, the CHD patients who were +294 C allele carriers had a 2.48-fold higher risk of LVH than subjects homozygous for the T allele. An increasing effect of the PPARD +294 C allele was shown on serum LDL-C levels in nondiabetic CHD patients. In addition, the results suggested that the +294 C allele might be associated with an increased LVH risk especially in male CHD patients. Furthermore, gene-gene interaction between the PPARD +294T/C and the APOE polymorphisms was observed regarding LDL-C concentrations.
- Subjects
TURKEY; CORONARY disease; GENETIC polymorphisms; HEART diseases; APOLIPOPROTEINS
- Publication
DNA & Cell Biology, 2012, Vol 31, Issue 5, p867
- ISSN
1044-5498
- Publication type
Article
- DOI
10.1089/dna.2011.1464