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- Title
A Novel GABA<sub>B</sub>R1a Receptor Electrochemical Biosensor Based on Gold Nanoparticles Chitosan‐horseradish Peroxidase.
- Authors
Zhao, Xiaotong; Wei, Lihui; Pang, Guangchang; Xie, Junbo
- Abstract
A novel gamma aminobutyric acid type B receptor subunit 1a (GABABR1a) biosensor modified with double‐layer gold has been developed for the first time. In this work, the synthesized plasmid was transfected into HEK‐293T cells by liposome transfection, and the target protein GABABR1a was obtained. The horseradish peroxidase was used as a signal amplification system to produce a double‐layer gold modified GABABR1a receptor biosensor. The ligand gamma aminobutyric acid (GABA), jujuboside A and baclofen were detected by the time‐current method with the biosensor. The results showed that the action regularity of GABA, jujuboside A, and baclofen on GABABR1a receptor was fitted with hyperbolic fitting (R2 was 0.9740, 0.9770 and 0.9770, respectively). The double reciprocal method was used to figure out the affinity constant (Ka) of GABA, jujuboside A, and baclofen with the receptor, i. e. 2.1016×10−15 mol/L, 1.7601×10−14 mol/L, and 1.633×10−14 mol/L, respectively, indicating that these measured compounds exhibited different action strength on the receptor. Based on the above, this study will provide a basis for drug screening and will also provide a new idea and method for investigating quantitatively the interaction between receptors and ligands in the future.
- Subjects
GABA; GOLD nanoparticles; BIOSENSORS; GLYCINE receptors; HORSERADISH peroxidase; PEROXIDASE; LIPOSOMES
- Publication
Electroanalysis, 2021, Vol 33, Issue 6, p1606
- ISSN
1040-0397
- Publication type
Article
- DOI
10.1002/elan.202060594