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- Title
Insight into the Effects of Adipose Tissue Inflammation Factors on miR-378 Expression and the Underlying Mechanism.
- Authors
Jiang, Xinye; Xue, Mei; Fu, Ziyi; Ji, Chenbo; Guo, Xirong; Zhu, Lu; Xu, Lulian; Pang, Lingxia; Xu, Meiyu; Qu, Hongming
- Abstract
Background/Aims: Obesity and the related metabolic syndrome have emerged as major public health issues in modern society. miRNAs have been shown to play key roles in regulating obesity-related metabolic syndrome, and some miRNAs regulated by adiponectin were identified as novel targets for controlling adipose tissue inflammation. miR-378 is a candidate target that was shown to be involved in adipose differentiation, mitochondrial metabolism and systemic energy homeostasis. However, little is known about the regulatory mechanisms of miR-378 expression. To better understand the physiological role of miR-378 in obesity and metabolic syndrome, it is crucial that we understand the regulation of miR-378 gene expression in human adipocytes. Methods: In this study, we investigated the effects of adipokines and inflammatory cytokines on miR-378 expression using Real-time PCR and the potential regulatory mechanisms using luciferase reporter assays and electrophoretic mobility shift assay (EMSA). Results: We found that adipokines and cytokines upregulated miR-378 expression primarily through SREBP and C/EBP binding sites in the miR-378 promoter region. Conclusion: Our findings showed that adipokines induced miR-378 expression and revealed the most likely mechanism of adipokine-induced miR-378 dysregulation in human adipocytes. miRNAs have been shown to function in regulating obesity-related metabolic syndrome, and miR-378 may be a novel target for controlling adipose tissue inflammation. This study offers a theoretical basis for understanding systemic adipose tissue inflammation and may provide new strategies for clinical treatment. © 2014 S. Karger AG, Basel
- Subjects
ADIPOSE tissues; INFLAMMATION; MICRORNA genetics; GENE expression; METABOLIC syndrome; PUBLIC health; CELL differentiation; CELLULAR control mechanisms; GENETICS
- Publication
Cellular Physiology & Biochemistry (Karger AG), 2014, Vol 33, Issue 6, p1778
- ISSN
1015-8987
- Publication type
Article
- DOI
10.1159/000362957