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- Title
制品中鸭源性成分 TaqMan 探针实时荧光 PCR 检测方法的建立与应用.
- Authors
刘立兵; 陈敏娜; 付 琦; 项佳林; 王金凤; 苗 丽; 孙晓霞; 艾连峰; 王建昌
- Abstract
Objective To establish a rapid detection method for duck-derived ingredients in meat products by a TaqMan real-time PCR assay. Methods Duck growth hormone (GH) gene was used as the target gene, and a real-time PCR method for the detection of duck-derived ingredients was established by designing primers and TaqMan probes based on specific conservative sequences and optimizing the reaction system and reaction conditions. Results The established real-time PCR method had strong specificity, only showed a specific amplification curve for duck genomic DNA, and had no amplification curve for genomic DNA of other heterologous animals such as cattle, sheep, pig, and so on. It had high sensitivity, using duck genomic DNA as template, and the detection limit was 1.0 pg; the repeatability was good, and the coefficient of variation of Ct value determined by different concentrations of genomic DNA was less than 4%. A total of 200 commercial meat products were detected using the established real-time PCR method, and duck-derived ingredients were detected in 11 meat products, which were consistent with the detection results in the current industry standard SN/T 3731.5—2013. Conclusion The real-time PCR method established in this study has the characteristics of strong specificity, high sensitivity, rapidity and high efficiency, and is suitable for rapid detection of duck-derived ingredients in meat products.
- Publication
Journal of Food Safety & Quality, 2021, Vol 12, Issue 8, p3048
- ISSN
2095-0381
- Publication type
Article