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- Title
Vancomycin-Loaded Collagen/Hydroxyapatite Layers Electrospun on 3D Printed Titanium Implants Prevent Bone Destruction Associated with S. epidermidis Infection and Enhance Osseointegration.
- Authors
Suchý, Tomáš; Vištejnová, Lucie; Šupová, Monika; Klein, Pavel; Bartoš, Martin; Kolinko, Yaroslav; Blassová, Tereza; Tonar, Zbyněk; Pokorný, Marek; Sucharda, Zbyněk; Žaloudková, Margit; Denk, František; Ballay, Rastislav; Juhás, Štefan; Juhásová, Jana; Klapková, Eva; Horný, Lukáš; Sedláček, Radek; Grus, Tomáš; Čejka Jr., Zdeněk
- Abstract
The aim of the study was to develop an orthopedic implant coating in the form of vancomycin-loaded collagen/hydroxyapatite layers (COLHA+V) that combine the ability to prevent bone infection with the ability to promote enhanced osseointegration. The ability to prevent bone infection was investigated employing a rat model that simulated the clinically relevant implant-related introduction of bacterial contamination to the bone during a surgical procedure using a clinical isolate of Staphylococcus epidermidis. The ability to enhance osseointegration was investigated employing a model of a minipig with terminated growth. Six weeks following implantation, the infected rat femurs treated with the implants without vancomycin (COLHA+S. epidermidis) exhibited the obvious destruction of cortical bone as evinced via a cortical bone porosity of up to 20% greater than that of the infected rat femurs treated with the implants containing vancomycin (COLHA+V+S. epidermidis) (3%) and the non-infected rat femurs (COLHA+V) (2%). The alteration of the bone structure of the infected COLHA+S. epidermidis group was further demonstrated by a 3% decrease in the average Ca/P molar ratio of the bone mineral. Finally, the determination of the concentration of vancomycin released into the blood stream indicated a negligible systemic load. Six months following implantation in the pigs, the quantified ratio of new bone indicated an improvement in osseointegration, with a two-fold bone ingrowth on the COLHA (47%) and COLHA+V (52%) compared to the control implants without a COLHA layer (27%). Therefore, it can be concluded that COLHA+V layers are able to significantly prevent the destruction of bone structure related to bacterial infection with a minimal systemic load and, simultaneously, enhance the rate of osseointegration.
- Subjects
OSSEOINTEGRATION; ORTHOPEDIC implants; HYDROXYAPATITE; OPERATIVE surgery; BONE growth; COMPACT bone
- Publication
Biomedicines, 2021, Vol 9, Issue 5, p531
- ISSN
2227-9059
- Publication type
Article
- DOI
10.3390/biomedicines9050531