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- Title
Quantitation of neurofilament light chain protein in serum and cerebrospinal fluid from patients with multiple sclerosis using the MSD R-PLEX NfL assay.
- Authors
Ulndreaj, Antigona; Sohaei, Dorsa; Thebault, Simon; Pons-Belda, Oscar D.; Fernandez-Uriarte, Amaia; Campbell, Christopher; Cheo, David; Stengelin, Martin; Sigal, George; Freedman, Mark S.; Scarisbrick, Isobel A.; Prassas, Ioannis; Diamandis, Eleftherios P.
- Abstract
Neurofilament light (NfL) chain is a marker of neuroaxonal damage in various neurological diseases. Here we quantitated NfL levels in the cerebrospinal fluid (CSF) and serum from patients with multiple sclerosis (MS) and controls, using the R-PLEX NfL assay, which employs advanced Meso Scale Discovery® (MSD) electrochemiluminescence (ECL)-based detection technology. NfL was quantitated in samples from 116 individuals from two sites (Ottawa Hospital Research Institute and Mayo Clinic), consisting of patients with MS (n=71) and age- and sex-matched inflammatory neurological controls (n=13) and non-inflammatory controls (n=32). Correlation of NfL levels between CSF and serum was assessed in paired samples in a subset of MS patients and controls (n=61). Additionally, we assessed the correlation between NfL levels obtained with MSD's R-PLEX® and Quanterix's single molecule array (Simoa®) assays in CSF and serum (n=32). Using the R-PLEX, NfL was quantitated in 99% of the samples tested, and showed a broad range in the CSF (82–500,000 ng/L) and serum (8.84–2,014 ng/L). Nf-L levels in both biofluids correlated strongly (r=0.81, p<0.0001). Lastly, Nf-L measured by MSD's R-PLEX and Quanterix's Simoa assays were highly correlated for both biofluids (CSF: r=0.94, p<0.0001; serum: r=0.95, p<0.0001). We show that MSD's R-PLEX NfL assay can reliably quantitate levels of NfL in the CSF and serum from patients with MS and controls, where levels correlate strongly with Simoa.
- Subjects
OTTAWA (Ont.); MAYO Clinic; CEREBROSPINAL fluid; BLOOD proteins; MULTIPLE sclerosis; CYTOPLASMIC filaments; NEUROLOGICAL disorders
- Publication
Diagnosis (2194-802X), 2023, Vol 10, Issue 3, p275
- ISSN
2194-802X
- Publication type
Article
- DOI
10.1515/dx-2022-0125