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- Title
F-FDG-labeled red blood cell PET for blood-pool imaging: preclinical evaluation in rats.
- Authors
Matsusaka, Yohji; Nakahara, Tadaki; Takahashi, Kazuhiro; Iwabuchi, Yu; Nishime, Chiyoko; Kajimura, Mayumi; Jinzaki, Masahiro
- Abstract
Background: Red blood cells (RBCs) labeled with single-photon emitters have been clinically used for blood-pool imaging. Although some PET tracers have been introduced for blood-pool imaging, they have not yet been widely used. The present study investigated the feasibility of labeling RBCs with F-2-deoxy-2-fluoro-D-glucose (F-FDG) for blood-pool imaging with PET. RBCs isolated from venous blood of rats were washed with glucose-free phosphate-buffered saline and labeled with F-FDG. To optimize labeling efficiency, the effects of glucose deprivation time and incubation (labeling) time with F-FDG were investigated. Post-labeling stability was assessed by calculating the release fraction of radioactivity and identifying the chemical forms of F in the released and intracellular components of F-FDG-labeled RBCs incubated in plasma. Just after intravenous injection of the optimized autologous F-FDG-labeled RBCs, dynamic PET scans were performed to evaluate in vivo imaging in normal rats and intraabdominal bleeding models (temporary and persistent bleeding). Results: The optimal durations of glucose deprivation and incubation (labeling) with F-FDG were 60 and 30 min, respectively. As low as 10% of F was released as the form of F-FDG from F-FDG-labeled RBCs after a 60-min incubation. Dynamic PET images of normal rats showed strong persistence in the cardiovascular system for at least 120 min. In the intraabdominal bleeding models, F-FDG-labeled RBC PET visualized the extravascular blood clearly and revealed the dynamic changes of the extravascular radioactivity in the temporary and persistent bleeding. Conclusions: RBCs can be effectively labeled with F-FDG and used for blood-pool imaging with PET in rats.
- Subjects
ERYTHROCYTES; PHOTONS; CARDIOVASCULAR system; RADIOACTIVITY; HEMORRHAGE
- Publication
EJNMMI Research, 2017, Vol 7, Issue 1, p1
- ISSN
2191-219X
- Publication type
Article
- DOI
10.1186/s13550-017-0266-3