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- Title
Enzymatic synthesis of theanine with Escherichia coli γ-glutamyltranspeptidase from a series of γ-glutamyl anilide substrate analogues.
- Authors
Zhang, Hong-juan; Zhang, Wei-guo; Wang, Zhi-yuan; Zhan, Yue-ping; Xu, Li-sheng; Liu, Jun-zhong; Liu, Qian; Jiao, Qing-cai
- Abstract
In order to investigate the catalytic mechanism of Escherichia coli γ-glutamyltranspeptidase, ten para- and meta-substituted γ-glutamyl anilides were chemically prepared and employed as substrates to synthesize L-theanine to assay the activity of γ-glutamyltranspeptidase. The reaction was optimized for γ-glutamyl-p-nitroanilide. Key factors such as substrate specificity, pH, temperature, and the substrate mole ratio were all investigated. Kinetic studies of the acyl transfer reaction were described and the Hammett plot was constructed. This study indicated that the ratelimiting acylation reaction of γ-glutamyltranspeptidase can apparently be accelerated by either the electron-withdrawing or electron-donating substituents of γ-glutamyl anilides. The reaction could be catalyzed by the general acid and carboxy of Asp-433 or phenolic hydroxyl Tyr-444 may be the acid by autodock simulation for all prepared γ-glutamyl anilides.
- Subjects
THEANINE; CATALYTIC activity; PEPTIDASE; ENZYME kinetics; ESCHERICHIA coli; NITROANILIDES; ACYLATION
- Publication
Biotechnology & Bioprocess Engineering, 2013, Vol 18, Issue 2, p358
- ISSN
1226-8372
- Publication type
Article
- DOI
10.1007/s12257-012-0644-7