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- Title
Molecular Prevalence of B artonella, B abesia, and Hemotropic M ycoplasma sp. in Dogs with Splenic Disease.
- Authors
Varanat, M.; Maggi, R.G.; Linder, K.E.; Breitschwerdt, E.B.
- Abstract
Background Among diseases that cause splenomegaly in dogs, lymphoid nodular hyperplasia ( LNH), splenic hemangiosarcoma ( HSA), and fibrohistiocytic nodules ( FHN) are common diagnoses. The spleen plays an important role in the immunologic control or elimination of vector-transmitted, blood-borne pathogens, including B artonella sp., B abesia sp., and hemotropic M ycoplasma sp. Objective To compare the prevalence of B artonella sp. , B abesia sp., and hemotropic M ycoplasma sp. DNA in spleens from dogs with LNH, HSA, and FHN. Materials and Methods Paraffin-embedded, surgically obtained biopsy tissues from LNH (N = 50), HSA (N = 50), and FHN (N = 37) were collected from the anatomic pathology archives. Spleens from specific pathogen-free ( SPF) dogs (N = 8) were used as controls. B artonella sp., B abesia sp., and M ycoplasma sp. DNA was amplified by PCR, followed by DNA sequencing. Results B artonella sp. DNA was more prevalent in FHN (29.7%) and HSA (26%) as compared to LNH (10%) ( P = .019, .0373, respectively) or control spleens (0.0%). The prevalence of B abesia sp. and hemotropic M ycoplasma sp. DNA was significantly lower than B artonella sp. DNA in HSA ( P = .0005, .006, respectively) and FHN ( P = .003, .0004, respectively). There was no statistically significant difference in DNA prevalence among the 3 genera in the LNH group. Conclusions The higher prevalence of B artonella sp. in FHN and HSA warrants future investigations to determine if this bacterium plays a role in the development of these splenic diseases.
- Subjects
BARTONELLA; BARTONELLA infections in animals; CANCER in animals; SPLEEN diseases; MACROPHAGES
- Publication
Journal of Veterinary Internal Medicine, 2011, Vol 25, Issue 6, p1284
- ISSN
0891-6640
- Publication type
Article
- DOI
10.1111/j.1939-1676.2011.00811.x