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- Title
Establishment of Deep-Eutectic-Solvent-Assisted Matrix Solid-Phase Dispersion Extraction for the Determination of Four Flavonoids in Scutellariae Radix Based on the Concept of Quality by Design.
- Authors
Fei Yang; Luyi Jiang; Hui Mao; Yanfang Zou; Chu Chu
- Abstract
Background: Sample preparation is the most crucial step in analytical schemes. Micro-matrix solid-phase dispersion, as a method for microextraction of analytes, has prevailed recently for its low sample and extraction solvent consumption. However, small amounts of adsorbent or sample, or a short extraction time, always bring uncertainty to the result when using this method. Objective: The aim was to develop a simple and reliable method of deep-eutectic-solvent-assisted ultrasonic-synchronized matrix solid-phase dispersion microextraction for the analysis of four flavonoids in Scutellariae Radix based on the concept of quality by design. Method: The ZSM-5 molecular sieve was used as a new adsorbent in the micro-matrix solid-phase dispersion process. Singlefactor and Box--Behnken designs were used to construct the design space. Results: Verification of the experiment demonstrated that the design space is robust. Under optimal conditions, all analytes showed good linearity (R² > 0.999), high reproducibility (RSD < 2.24%) and stability (RSD < 2.87%), and satisfactory recoveries (95.90--102.31%), which indicated that the established method is reliable and reproducible. Moreover, it has been successfully applied to determine the flavonoids in nine batches of Scutellariae Radix. Conclusions: The results indicate a great potential for analyzing complicated samples especially with small amount and helping to promote the quality control of the sample preparation process for traditional Chinese medicines. Highlights: A systematic approach using a facile deep-eutectic-solvent-assisted ultrasonic-synchronized matrix solid-phase dispersion extraction coupled with HPLC for the analysis of flavonoids in Scutellariae Radix has been developed based on the concept of quality by design.
- Subjects
SOLID phase extraction; FLAVONOIDS; CHINESE medicine; PARTICLE size determination; MOLECULAR sieves; SOLVENT extraction
- Publication
Journal of AOAC International, 2021, Vol 104, Issue 6, p1681
- ISSN
1060-3271
- Publication type
Article
- DOI
10.1093/jaoacint/qsab043