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- Title
HPTLC Determination of Artesunate as Bulk Drug and in Pharmaceutical Formulations.
- Authors
Agarwal, S. P.; Ali, A.; Ahuja, Shipra
- Abstract
A new, simple, rapid, accurate and precise HPTLC method has been developed for the estimation of artesunate in bulk and pharmaceutical formulations. The study employs silica gel F254 as stationary phase on aluminium foil and mobile phase comprising toluene: ethyl acetate: acetic acid (2:8:0.2). Vanillin (1%) in sulphuric acid (5%) in ethanolic solution gave prominent well-resolved pink colour spot for artesunate, which was stable for more than a day. The densitometric analysis was carried out in the absorbance mode at 520 nm and symmetrical, well-resolved, well-defined peaks were obtained. The Rf value for artesunate was found to be 0.44. The linear detector response for artesunate was observed between 100-600 ng per spot and the calibration plots showed good linear relationship with coefficient of regression, r= 0.9989 with respect to peak area. The method was validated for precision, recovery and robustness. The limits of detection and quantitation were 30 ng/spot and 90 ng/spot, respectively. The recovery study was carried out by standard addition method and the recovery was found to be 99.89±1.006. Recovery from tablets was 98.88 (±0.55) and from injection, it was 98.83 (±0.60) of the labeled amount.
- Subjects
HIGH performance liquid chromatography; ANTIMALARIALS; DOSAGE forms of drugs; DRUG analysis; PHARMACOLOGY
- Publication
Indian Journal of Pharmaceutical Sciences, 2007, Vol 69, Issue 6, p841
- ISSN
0250-474X
- Publication type
Article
- DOI
10.4103/0250-474X.39450