We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Antimetastatic activity of a cyclooxygenase-2 inhibitor.
- Authors
Roche-Nagle, G; Connolly, EM; Eng, M; Bouchier-Hayes, DJ; Harmey, JH; Connolly, E M; Bouchier-Hayes, D J; Harmey, J H
- Abstract
Cyclooxygenase-2 (COX-2) expression is increased in breast cancer and surgery has been shown to increase the growth of metastatic tumours. We investigated the effect of selective COX-2 inhibition on the growth of metastases in either an experimental metastasis model or following excision of a murine primary breast tumour. 50,000 4T1 mammary carcinoma cells were injected into the mammary fat pad of female BALB/c mice. When the mean TD reached 8+/-0.4 mm, tumours were excised and the mice were randomised into two groups (n=12 per group) to receive daily intraperitoneal injections of the selective COX-2 inhibitor, SC-236 or drug vehicle for 14 days. Alternatively, experimental metastases were established by tail-vein injection of 50,000 4T1 cells. Mice received either the selective COX-2 inhibitor, SC-236 or drug vehicle for 14 days (n=12 per group). SC-236 treatment significantly reduced tumour burden, the number and size of spontaneous metastases following primary tumour excision. SC-236 treatment also reduced tumour burden, the number and size of experimental metastases. Immunohistochemical staining demonstrated that COX-2 inhibition reduced microvessel density and increased apoptosis within both spontaneous and experimental metastases. These data clearly demonstrate that the selective COX-2 inhibitor, SC-236, has potent antimetastatic activity against both spontaneous metastases arising following primary tumour excision and experimental metastases.
- Subjects
CYCLOOXYGENASE 2; BREAST cancer; SURGERY; METASTASIS; PATHOLOGY; ONCOLOGY; BREAST tumor treatment; ADENOCARCINOMA; RESEARCH; HETEROCYCLIC compounds; NONSTEROIDAL anti-inflammatory agents; ANIMAL experimentation; RESEARCH methodology; APOPTOSIS; EVALUATION research; ISOENZYMES; COMPARATIVE studies; PATHOLOGIC neovascularization; OXIDOREDUCTASES; ENZYME inhibitors; SULFONAMIDES; BREAST tumors; MICE; CHEMICAL inhibitors
- Publication
British Journal of Cancer, 2004, Vol 91, Issue 2, p359
- ISSN
0007-0920
- Publication type
journal article
- DOI
10.1038/sj.bjc.6601967