We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Transfection of interleukin-8 increases angiogenesis and tumorigenesis of human gastric carcinoma cells in nude mice.
- Authors
Kitadai, Y; Takahashi, Y; Haruma, K; Naka, K; Sumii, K; Yokozaki, H; Yasui, W; Mukaida, N; Ohmoto, Y; Kajiyama, G; Fidler, I J; Tahara, E
- Abstract
The growth and spread of tumour cells depends on adequate vasculature. We have previously reported that the expression of interleukin-8 (IL-8) directly correlates with the vascularity of human gastric carcinomas. To provide evidence for a causal role of IL-8 in angiogenesis and tumorigenicity of human gastric cancer, we used the lipofectin method to stably transfect the human TMK-1 gastric carcinoma cells (low endogenous IL-8) with an IL-8 expression vector or control vector. Transfection with IL-8 did not affect the proliferation of cultured cells, yet the culture supernatants of the transfected (but not control) cells stimulated proliferation of human umbilical vein endothelial cells. The IL-8-transfected and control cells were injected into the gastric wall of nude mice. IL-8-transfected cells produced rapidly growing, highly vascular neoplasms as compared to control cells. These results provide direct evidence for the role of IL-8 in the angiogenesis and tumorigenicity of human gastric carcinomas.
- Subjects
INTERLEUKIN-8; NEOVASCULARIZATION
- Publication
British Journal of Cancer, 1999, Vol 81, Issue 4, p647
- ISSN
0007-0920
- Publication type
journal article
- DOI
10.1038/sj.bjc.6690742