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- Title
Mechanical loading of tissue engineered skeletal muscle prevents dexamethasone induced myotube atrophy.
- Authors
Aguilar-Agon, Kathryn W.; Capel, Andrew J.; Fleming, Jacob W.; Player, Darren J.; Martin, Neil R. W.; Lewis, Mark P.
- Abstract
Skeletal muscle atrophy as a consequence of acute and chronic illness, immobilisation, muscular dystrophies and aging, leads to severe muscle weakness, inactivity and increased mortality. Mechanical loading is thought to be the primary driver for skeletal muscle hypertrophy, however the extent to which mechanical loading can offset muscle catabolism has not been thoroughly explored. In vitro 3D-models of skeletal muscle provide a controllable, high throughput environment and mitigating many of the ethical and methodological constraints present during in vivo experimentation. This work aimed to determine if mechanical loading would offset dexamethasone (DEX) induced skeletal muscle atrophy, in muscle engineered using the C2C12 murine cell line. Mechanical loading successfully offset myotube atrophy and functional degeneration associated with DEX regardless of whether the loading occurred before or after 24 h of DEX treatment. Furthermore, mechanical load prevented increases in MuRF-1 and MAFbx mRNA expression, critical regulators of muscle atrophy. Overall, we demonstrate the application of tissue engineered muscle to study skeletal muscle health and disease, offering great potential for future use to better understand treatment modalities for skeletal muscle atrophy.
- Subjects
MUSCULAR atrophy; SKELETAL muscle; TISSUE engineering; MUSCULAR dystrophy; MUSCLE weakness; ATROPHY
- Publication
Journal of Muscle Research & Cell Motility, 2021, Vol 42, Issue 2, p149
- ISSN
0142-4319
- Publication type
Article
- DOI
10.1007/s10974-020-09589-0