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- Title
The effects of case/control ratio and sample size on genome‐wide association studies: A simulation study.
- Authors
Turgut, Ali Osman; Koca, Davut
- Abstract
Background: Genome‐wide association studies (GWAS) is a useful tool for the detection of disease or quantitative trait‐related genetic variations in the veterinary field. For a binary trait, a case/control experiment is designed in GWAS. However, there is limited information on the optimal case/control and sample size in GWAS. Objectives: In this study, it was aimed to detect the effects of case/control ratio and sample size for GWAS using computer simulation under certain assumptions. Method: Using the PLINK software, we simulated three different disease scenarios. In scenario 1, we simulated 10 different case/control ratios with increasing ratio of cases to controls. In scenario 2, we did versa of scenario 1 with the increasing ratio of controls to cases. In scenarios 1 and 2, sample size gradually was increased with the change case/control ratios. In scenario 3, the total sample size was fixed to 2000 to see real effects of case/control ratio on the number of disease‐related single nucleotide polymorphisms (SNPs). Results: The results showed that the number of disease‐related SNPs were the highest when the case/control ratio is close to 1:1 in scenarios 1 and 2 and did not change with an increase in sample size. Similarly, the number of disease‐related SNPs was the highest in case/control ratios 1:1 in scenario 3. However, unbalanced case/control ratio caused the detection of lower number of disease‐related SNPs in scenario 3. The estimated average power of SNPs was highest when case/control ratio is 1:1 in all scenarios. Conclusions: All findings led to the conclusion that an increase in sample size may enhance the statistical power of GWAS when the number of cases is small. In addition, case/control ratio 1:1 may be the optimal ratio for GWAS. These findings may be valuable not only for veterinary field but also for human clinical experiments.
- Subjects
GENOME-wide association studies; SAMPLE size (Statistics); SINGLE nucleotide polymorphisms; GENETIC variation; STATISTICAL power analysis
- Publication
Veterinary Medicine & Science, 2024, Vol 10, Issue 3, p1
- ISSN
2053-1095
- Publication type
Article
- DOI
10.1002/vms3.1444