We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Evaluation of New Generation Sequencing (NGS)-Based Somatic Gene Variations and Real-Time Polymerase Chain Reaction (PCR)-Based Gene Fusions in Elderly and Young Acute Leukemia Patients: A Retrospective View.
- Authors
Erdoğdu, İbrahim Halil; Örenay-Boyacıoğlu, Seda; Boyacıoğlu, Olcay; Kahraman-Çetin, Nesibe; Kacar-Döger, Füruzan; Yavaşoğlu, İrfan; Bolaman, Ali Zahit
- Abstract
Malignant diseases occurring in elderly patients follow a different course from younger patients and show different genetic structures. Therefore, in this retrospective study, the somatic gene variant profile and fusion gene profiles of elderly and young acute leukemia patients were determined to draw attention to the existing genetic difference, and the results were compared. In this study, the records of 204 acute leukemia patients aged 18+ who were referred to the Molecular Pathology Laboratory from the Hematology Clinic between 2018 and 2022 were reviewed retrospectively. Fusion gene detection in patients was performed with the HemaVision®-28Q Panel. The NGS Myeloid Neoplasms Panel was conducted using the MiniSEQ NGS platform according to the manufacturer's protocol. When all cases are evaluated together, the most frequently diagnosed acute leukemia is acute myeloid leukemia (85.8%). Both groups had a similar fusion gene profile; however, the fusion burden was higher in the elderly group. When the groups were evaluated in terms of somatic gene variations, there were differences between the groups, and the variation load was higher in the elderly group. Considering the different somatic gene variation profiles, it is understood that the genetic structure of tumor cells is different in elderly patients compared to young cases.
- Subjects
ACUTE leukemia; GENE fusion; POLYMERASE chain reaction; OLDER patients; OLDER people
- Publication
Journal of Personalized Medicine, 2024, Vol 14, Issue 2, p140
- ISSN
2075-4426
- Publication type
Article
- DOI
10.3390/jpm14020140