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- Title
Identification of differentially expressed genes in longissimus muscle of pigs with high and low intramuscular fat content using RNA sequencing.
- Authors
Lim, K. S.; Lee, K. T.; Park, J. E.; Chung, W. H.; Jang, G. W.; Choi, B. H.; Hong, K. C.; Kim, T. H.
- Abstract
Intramuscular fat ( IMF) content in pork is an important element of consumer preference and is positively correlated with meat quality, including tenderness and juiciness. With advances in RNA sequencing technologies, transcriptome-related differences can be associated with specific traits in animals. The objective of this study was to investigate differentially expressed genes ( DEGs) closely related to IMF content in porcine longissimus muscle using RNA sequencing. A total of 107 Berkshire pigs were used for IMF content measurements, and significant differences between extremely high (H, n = 3) and low (L, n = 3) IMF content groups were found ( P < 0.0001). From multi-dimensional scaling analyses, it was observed that the relationships between H and L groups were similar to each other. Here, we identified a total of 134 genes that were differentially expressed between the groups (false discovery rate <0.05; fold change ≥2). Functional analyses with DEGs revealed that lipid metabolism ( SCD and FASN) was one of the significant biological processes related to IMF content determination. In addition, we found that DEGs related to muscle regeneration ( MYOG and VEGFA) and extracellular matrix ( COL1A1, COL1A2, COL5A1, COL14A1 and COL15A1) were changed among individuals with extreme IMF contents. These results will aid in understanding the regulation of IMF content in pigs.
- Subjects
ERECTOR spinae muscles; SWINE; RNA sequencing; GENE ontology; LIPID metabolism
- Publication
Animal Genetics, 2017, Vol 48, Issue 2, p166
- ISSN
0268-9146
- Publication type
Article
- DOI
10.1111/age.12518