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- Title
New insights in extracellular matrix remodeling and collagen turnover related pathways in cultured human tenocytes after ciprofloxacin administration.
- Authors
Menon, Alessandra; Pettinari, Letizia; Martinelli, Carla; Colombo, Graziano; Portinaro, Nicola; Dalle-Donne, Isabella; d'Agostino, Maria Cristina; Gagliano, Nicoletta
- Abstract
We characterized the effect of ciprofloxacin (CPX) in cultured human tenocytes by morphological and molecular methods. Collagen type I and III mRNA and protein levels were unaffected, but lysyl hydroxylase 2b mRNA levels progressively decreased after CPX administration. MMP-1 protein levels significantly increased after 20 μg/ml CPX administration but remained unmodified at the higher dose, whilst MMP-2 activity was unchanged. Tissue inhibitor of MMP (TIMP-1) gene expression decreased after CPX treatment, whilst TIMP-2 and transforming growth factor-β1 gene expression, the cytoskeleton arrangement, and cytochrome c expression remained unmodified. Secreted Protein Acidic and Rich in Cysteine mRNA and protein levels remained almost unchanged, whilst N-cadherin mRNA levels resulted significantly down-regulated and connexin 43 gene expression tended to decrease after CPX administration. The CPX-induced decreased ability to cross-link collagen and decreased TIMP-1 levels, possibly leading to higher activity of MMPs in ECM degradation, together with the down-regulation of N-cadherin and connexin 43 are consistent with a reduced ability to maintain tissue homeostasis, possibly making the tendon more susceptible to rupture.
- Subjects
ANALYSIS of variance; CELLULAR signal transduction; CIPROFLOXACIN; COLLAGEN; EXTRACELLULAR space; GENE expression; RESEARCH funding; STATISTICS; TENDONS; DATA analysis; DESCRIPTIVE statistics; SEQUENCE analysis; PHARMACODYNAMICS
- Publication
Muscles, Ligaments & Tendons Journal (MLTJ), 2013, Vol 3, Issue 3, p122
- ISSN
2240-4554
- Publication type
Article