We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Activation of Eukaryotic Transcriptional Promoters by the Bovine Papillomavirus E1-Replication Factor.
- Authors
Monini, Paolo; de Lellis, Laura; Borgatti, Paola; Hassan-Omran, Moataza; Cassai, Enzo
- Abstract
It has been suggested that the bovine papillomavirus type 1 E1 replication factor may inhibit E2-conditional gene expression from viral promoter P89. To study the possible role of the E1 protein in gene expression, HeLa cells were transfected with E2-conditional or E2-independent reporter plasmids and with vectors expressing the E1 and E2 open reading frames. The data show that: (i) replication factor E1 stimulates gene expression from a variety of eucaryotic transcriptional promoters; (ii) activation of gene expression by E1 also occurs in the absence of viral activator E2-TA; (iii) expression of a recombinant plasmid containing the human papillomavirus type 18 origin of DNA replication is stimulated, but not repressed, by E1, and (iv) E1-dependent activation of gene expression does not reflect the amplification of transfected plasmids. Copyright © 1993 S. Karger AG, Basel
- Publication
Intervirology, 1993, Vol 36, Issue 4, p245
- ISSN
0300-5526
- Publication type
Article
- DOI
10.1159/000150343