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- Title
Réliability of In-Situ Hybridization of Smears and Biopsies for Papilloma Virus Genotyping of the Uterine Cervix.
- Authors
Czerwenka, K. F.; Schön, H. J.; Manavi, M.; Zeillinger, R.; Kubista, E.; Gitsch, E.
- Abstract
Two sampling methods, biopsy and cell smear, were investigated for their reliability in the biotin in-situ hybridization test for human papilloma virus deoxyribonucleic acid (HPV-DNA)-6/ll and 16/18. Cervical smears and biopsies were obtained simultaneously from 81 women with cervical lesions. The sensitivity of the in-situ hybridization was tested on human cervical carcinoma cell lines. As a reference method, HPVDNA was probed in biopsies using a Southern blot with 32P-labelled DNA probes. This reference method detected HPV-DNA in 45% of the reactive and reparative cell changes, in 75.9% of intraepithelial neoplasias, and in 83.3% of squamous cell carcinomas of the cervix. Of the examined cervices 61.7% were HPV-DNApositive. As tested by in-situ hybridization, 56.8% of the biopsies were HPV-DNA-positive. Three biopsies were HPVDNA- negative by the in-situ hybridization but positive by the Southern blot. One biopsy was HPV-DNA- 6/11 positive by the in-situ hybridization but negative in the Southern blot. As tested by in-situ hybridization, 55.6% of the cervical smears were HPV-DNA-positive. Five smears were HPV-DNA-positive by the in-situ hybridization but negative by the reference method, thus demonstrating the dependence of the HPV positive rate on the sampling method. Four cell smears were negative by the insitu hybridization but positive by the reference method, which shows that the biotin in-situ hybridization is less sensitive. The reference method confirmed the HPV-DNA-positivity of in-situ hybridization for 97.8% of biopsies, and for 91.8% of cell smears. Compared with the reference method the diagnostic efficiency of the in-situ hybridization is 98.9% for biopsies, and 96.9% for cell smears. The in-situ hybridization therefore represents a reliable technique for viral genotyping in cervical specimens.
- Publication
Clinical Chemistry & Laboratory Medicine, 1991, Vol 29, Issue 2, p139
- ISSN
1434-6621
- Publication type
Article