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- Title
Identification of a Novel Hepatitis C Virus Genotype From Punjab, India: Expanding Classification of Hepatitis C Virus Into 8 Genotypes.
- Authors
Borgia, Sergio M; Hedskog, Charlotte; Parhy, Bandita; Hyland, Robert H; Stamm, Luisa M; Brainard, Diana M; Subramanian, Mani G; McHutchison, John G; Mo, Hongmei; Svarovskaia, Evguenia; Shafran, Stephen D
- Abstract
<bold>Background: </bold>Hepatitis C virus (HCV) exhibits great genetic diversity and is classified into 7 genotypes (GTs), with varied geographic prevalence. Until the recent development of pangenotypic direct-acting antiviral regimens, the determination of HCV GT was necessary to inform optimal treatment.<bold>Methods: </bold>Plasma samples with unresolved GT using standard commercial genotyping methods were subjected to HCV full-genome sequencing, and phylogenetic analysis was performed to assign GT.<bold>Results: </bold>Four patients, previously classified as GT5 by LiPA or Abbott RealTime polymerase chain reaction assays, were identified as infected with a novel HCV GT. This novel HCV GT, GT8, is genetically distinct from previously identified HCV GT1-7 with >30% nucleotide sequence divergence to the established HCV subtypes. All 4 patients were originally from Punjab, India, but now reside in Canada and are epidemiologically unlinked. Despite presence of baseline resistance-associated substitutions within the GT8 virus of all 4 patients (NS3: V36L, Q80K/R; NS5A: Q30S, Y93S), all patients achieved a sustained virologic response; 2 treated with sofosbuvir/velpatasvir/voxilaprevir for 8 weeks, 1 with sofosbuvir/ledipasvir plus ribavirin for 24 weeks and 1 with sofosbuvir plus daclatasvir for 12 weeks.<bold>Conclusions: </bold>The discovery of a novel HCV GT8 confirms the circulation of this newly identified lineage in the human population.
- Subjects
INDIA; HEPATITIS C virus; GENOTYPES; ANTIVIRAL agents; NUCLEOTIDE sequencing; RIBAVIRIN; CLINICAL trials; DRUG resistance in microorganisms; BIOLOGICAL evolution; GENETICS; GENOMES; HEPATITIS C; HEPATITIS viruses; RNA; GENOMICS; SEQUENCE analysis; PHARMACODYNAMICS
- Publication
Journal of Infectious Diseases, 2018, Vol 218, Issue 11, p1722
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiy401