We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
FAM134B, the Selective Autophagy Receptor for Endoplasmic Reticulum Turnover, Inhibits Replication of Ebola Virus Strains Makona and Mayinga.
- Authors
Chiramel, Abhilash I.; Dougherty, Jonathan D.; Nair, Vinod; Robertson, Shelly J.; Best, Sonja M.
- Abstract
Selective autophagy of the endoplasmic reticulum (termed ER-phagy) is controlled by members of the FAM134 reticulon protein family. Here we used mouse embryonic fibroblasts from mice deficient in FAM134B to examine the role of the ER in replication of historic (Mayinga) or contemporary (Makona GCO7) strains of Ebola virus (EBOV). Loss of FAM134B resulted in 1-2 log10 higher production of infectious EBOV, which was associated with increased production of viral proteins GP and VP40 and greater accumulation of nucleocaspid lattices. In addition, only 10% of wild-type cells contained detectable nucleoprotein, whereas knockout of FAM134B resulted in 80% of cells positive for nucleoprotein. Together, these data suggest that FAM134B-dependent ER-phagy is an important limiting event in EBOV replication in mouse cells and may have implications for further development of antiviral therapeutics and murine models of infection.
- Subjects
TREATMENT of Ebola virus diseases; AUTOPHAGY; ENDOPLASMIC reticulum; VIRAL replication; ANTIVIRAL agents; NUCLEOPROTEINS
- Publication
Journal of Infectious Diseases, 2016, Vol 214, pS319
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1093/infdis/jiw270