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- Title
Prognostic and diagnostic potential of miR-146a in oesophageal squamous cell carcinoma.
- Authors
Wang, Cong; Guan, Shanghui; Liu, Fang; Chen, Xuan; Han, Lihui; Wang, Ding; Nesa, Effat Un; Wang, Xintong; Bao, Cihang; Wang, Nana; Cheng, Yufeng
- Abstract
<bold>Background: </bold>Accumulating evidence indicates that dysregulated microRNA-146a (miR-146a) is involved in tumour genesis and cancer progression. We aimed to evaluate its expression level and the potential for the diagnosis and prognosis in oesophageal squamous cell cancer (ESCC).<bold>Methods: </bold>We examined miR-146a expression in 62 pairs of ESCC cancerous and matched paracancerous tissue, 115 formalin-fixed paraffin-embedded (FFPE) tissue samples and serum samples from 154 ESCC patients and 154 healthy volunteers using quantitative reverse transcription-PCR (qRT-PCR). Kaplan-Meier method, Cox regression and receiver-operating characteristic (ROC) curve analysis were applied to analyse its prognostic and diagnostic value.<bold>Results: </bold>MicroRNA-146a expression level was significantly decreased in ESCC tissue compared with paracancerous tissue (P<0.001). Its regulation level was negatively associated with T factor and TNM stage. Kaplan-Meier curve revealed that its downregulation level predicted worse overall survival (OS) and progression-free survival (PFS). Both univariate and multivariate analyses identified miR-146a expression as independent prognostic factor for OS and PFS. Serum miR-146a was significantly reduced in ESCC patients than in healthy controls (P<0.001). Area under the curve ROC value, sensitivity and specificity for this marker were 0.863 ± 0.033, 85.7% and 68.6% in the Discovery Group, and 0.891 ± 0.027, 82.1% and 83.3% in the Validation Group.<bold>Conclusions: </bold>MicroRNA-146a is significantly reduced in cancerous tissue and serum samples of ESCC patients. It is an ideal biomarker for the prognosis and diagnosis of ESCC.
- Publication
British Journal of Cancer, 2016, Vol 114, Issue 3, p290
- ISSN
0007-0920
- Publication type
journal article
- DOI
10.1038/bjc.2015.463