We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Analysis of gene alterations of mitochondrial DNA D-loop regions to determine breast cancer clonality.
- Authors
Masuda S; Kadowaki T; Kumaki N; Tang X; Tokuda Y; Yoshimura S; Takekoshi S; Osamura RY; Masuda, S; Kadowaki, T; Kumaki, N; Tang, X; Tokuda, Y; Yoshimura, S; Takekoshi, S; Osamura, R Y
- Abstract
<bold>Background: </bold>It has been a challenge to determine breast cancer clonality accurately. The aim of the present study was to assess methods using formalin-fixed paraffin-embedded (FFPE) tissue to differentiate new primary tumours from true recurrences that are associated with poorer prognoses and often require more aggressive treatment.<bold>Methods: </bold>We investigated the novel method of analysing gene alterations of mitochondrial DNA D-loop region (GAMDDL) and compared it with the conventional method of analysing the X-chromosome-linked human androgen receptor (HUMARA). The FFPE sections of primary and secondary breast cancers, the non-neoplastic mammary gland, and lymph nodes were examined.<bold>Results: </bold>Informative rates for HUMARA, GAMDDL, and combined analyses were 42.1%, 76.9%, and 89.5%, respectively. All of the 10 contralateral breast cancers were determined to be non-clonal. In contrast, 3 out of 8 (37.5%) of the ipsilateral secondary tumours shared a clonal origin with the primary tumour and were classified as true recurrences, whereas 4 out of 8 (50%) were classified as new primary tumours.<bold>Conclusion: </bold>GAMDDL analysis represents a novel and useful molecular method for examining the precise cell lineages of primary and secondary tumours, and was more accurate than HUMARA in determining clonality.
- Publication
British Journal of Cancer, 2012, Vol 107, Issue 12, p2016
- ISSN
0007-0920
- Publication type
journal article
- DOI
10.1038/bjc.2012.505