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- Title
Identification of the ligand of Pru p 3, a peach LTP.
- Authors
Cubells-Baeza, Nuria; Gómez-Casado, Cristina; Tordesillas, Leticia; Ramírez-Castillejo, Carmen; Garrido-Arandia, María; González-Melendi, Pablo; Herrero, María; Pacios, Luis; Díaz-Perales, Araceli
- Abstract
Key message: Pru p 3, a peach LTP, is located in pollinated flower styles and secreting downy hairs, transporting a derivative of camptothecin bound to phytosphingosine. Pru p 3 may inhibit a second pollination and may keep away herbivores until seed maturation. Abstract: The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein. In addition, the described ligand displayed topoisomerase I activity inhibition and self-fluorescence, both recognized as camptothecin properties. During flower development, the highest expression of Pru p 3 was detected in the styles of pollinated flowers, in contrast to its non-expression in unpollinated pistils, where expression decreased after anthesis. During ripening, the expression of Pru p 3 were observed mainly in peel but not in pulp. In this sense, Pru p 3 protein was also localized in trichomes covering the fruit epidermis.
- Subjects
LIPID transfer protein; LIGANDS (Biochemistry); CAMPTOTHECIN; ALLERGENS; POLLINATION; FRUIT development
- Publication
Plant Molecular Biology, 2017, Vol 94, Issue 1-2, p33
- ISSN
0167-4412
- Publication type
Article
- DOI
10.1007/s11103-017-0590-z