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- Title
False negative factor V Leiden assay following allogeneic stem cell transplant.
- Authors
Crookston, Kendall P.; Henderson, Ruth; Chandler, Wayne L.
- Abstract
We report a case where a phenotypic test (an activity assay for activated protein C resistance) correctly indicated that the patient had an abnormality, whereas the initial genetic test (a PCR-based DNA assay used to detect the mutation in the FV gene) incorrectly indicated that the patient did not. The apparent false negative result of the DNA-based test was due to the use of peripheral blood leucocytes for DNA analysis. The patient had undergone a stem cell transplant several months before, and the leucocytes in her blood were derived from the stem cell donor, which lacked the FV defect.
- Subjects
GENES; TRANSPLANTATION immunology; CLASSIFICATION
- Publication
British Journal of Haematology, 1998, Vol 100, Issue 3, p600
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1046/j.1365-2141.1998.00585.x