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- Title
Hydrolyzed Conchiolin Protein Inhibits Melanogenesis by Increasing miR-26a-1-3p in Melanoma Cells.
- Authors
YAQI ZHANG; SISI WANG; ANQUAN YANG; CHENGLONG YE; ZHE LI; LONG ZHU
- Abstract
Hydrolyzed conchiolin protein (HCP) can act as a cosmetic skin-whitening agent by inhibiting melanogenesis through effects on the enzyme tyrosinase (TYR) and reducing melanin production in B16F10 melanoma cells. However, details of the molecular mechanism underlying this effect have not been fully elucidated. miRNA is a type of ncRNA of approximately 21-25 nucleotides that exhibits binding affinity toward a specific region (r epresented by the 3' untranslated region [UTR]) of mu ltiple mRNA target molecules. We investigated the effects of HCP on the miR-26a-1-3p, which is predicted to bind the upstream regulator of TYR, microphthalmia-associated transcription factor (MITF), in B16F10 melanoma cells. We assessed whether miR-26a-1-3p regulates MITF and indirectly affects the pathways of TYR and TYR-related protein-1 (TRP-1) expression levels, thereby influencing melanogenesis. The 3' UTR of MITF was verified to be a miR-26a-1-3p t arget gene by bioinformatics prediction and a dual-luciferase reporter assay. Overexpression of miR-26a-1-3p in cultured cells decreased MITF, TYR, and TYR-related protein-1 (TRP-1) expression levels. HCP treatment increased miR-26a-1-3p levels, inhibiting MITF and leading to decreased TYR and TRP-1 levels. Our results suggest that HCP controls melanin production by increasing miR-26a-1-3p levels and inhibiting MITF, TYR, and TRP-1 expression, indicating its potential as a whitening product.
- Publication
Journal of Cosmetic Science, 2024, Vol 75, Issue 3, p174
- ISSN
1525-7886
- Publication type
Article