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- Title
Evaluation of cross-protection with an attenuated isolate ofBean yellow mosaic virusby differential detection of virus isolates using RT-PCR.
- Authors
Nakazono-Nagaoka, Eiko; Sato, Chikako; Kosaka, Yoshitaka; Natsuaki, Tomohide
- Abstract
Cross-protection by an attenuated severe isolate ofBean yellow mosaic virus(BYMV), a member of genusPotyvirus, was evaluated. The attenuated isolate, M11, produced no or extremely mild symptoms on broad bean andNicotiana benthamiana. To differentiate between M11 and severe isolates, we prepared specific primers for analysis by the reverse transcription-polymerase chain reaction (RT-PCR) based on a difference in the nucleotide sequence at the 1676th position in theHC-Pro(helper component proteinase) gene. M11 had a strong ability to cross-protect plants from infection by a severe isolate as shown by RT-PCR. Plants that were first inoculated with a severe isolate and then challenge-inoculated with M11 were dually infected by these viruses, and the symptoms on upper leaves were significantly milder than those on plants infected only with the severe isolate.
- Subjects
BEAN yellow mosaic virus; POTYVIRUS diseases; PLANT viruses; PATHOGENIC microorganisms; PLANT diseases
- Publication
Journal of General Plant Pathology, 2004, Vol 70, Issue 6, p359
- ISSN
1345-2630
- Publication type
Article
- DOI
10.1007/s10327-004-0138-3