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- Title
Sulfated polysaccharide ascophyllan prevents amyloid fibril formation of human insulin and inhibits amyloid-induced hemolysis and cytotoxicity in PC12 cells.
- Authors
Yan Liang; Mikinori Ueno; Shijiao Zha; Takasi Okimura; Zedong Jiang; Kenichi Yamaguchi; Tomomitsu Hatakeyama; Tatsuya Oda
- Abstract
We found that ascophyllan significantly inhibited the fibrillation of human insulin and was the most effective among the sulfated polysaccharides tested. Gel-filtration analysis suggested that ascophyllan was capable of forming a complex with insulin through a weak interaction. Secondary structure transition from native α-helix to β-sheet predominant structure of insulin under the fibrillation conditions was suppressed in the presence of ascophyllan. Interestingly, ascophyllan attenuated insulin fibril-induced hemolysis of human erythrocytes. Moreover, ascophyllan attenuated insulin amyloid-induced cytotoxicity on rat pheochromocytoma PC12 cells and reduced the level of intracellular reactive oxygen species. This is the first report indicating that a sulfated polysaccharide, ascophyllan, can suppress the insulin amyloid fibril formation and inhibit the fibril-induced detrimental bioactivities. Ascophyllan can suppress the insulin amyloid fibril formation.
- Subjects
INSULIN; AMYLOID; AMYLOID beta-protein; HEMOLYSIS &; hemolysins; REACTIVE oxygen species
- Publication
Bioscience, Biotechnology & Biochemistry, 2021, Vol 85, Issue 11, p2281
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbab163