We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Characterization of collagenase found in the nonpathogenic bacterium Lysinibacillus sphaericus VN3.
- Authors
Hoa Bach, Thi Mai; Pham, Thanh Huyen; Dinh, Truong Son; Takagi, Hiroshi
- Abstract
High collagenolytic activity has been detected in pathogenic bacteria. Collagenase plays an essential role in the invasion step in animals and humans. In this study, we characterized collagenase found in the nonpathogenic bacterium Lysinibacillus sphaericus VN3, which was isolated from soil in Vietnam. The collagenase activity of the purified enzyme was strongly inhibited by Cu2+, but it was significantly increased by Zn2+. The purified enzyme with a molecular mass of approximately 110 kDa exhibited collagenolytic, gelatinolytic, and caseinolytic activity. The kinetic studies showed that this enzyme had greater hydrolyzing activity toward collagen and gelatin compared with casein. Based on the ratio Vmax/Km, collagen is likely to be the best substrate among three proteins. We found that this collagenase could digest small pieces of bovine skin and tendon into a collagen solution. Interestingly, at pH 6.0–8.0, the soluble collagen could form a collagen membrane, which is useful as a wound-healing biomaterial. New collagenase found in the soil bacterium Lysinibacillus sphaericus VN3 could form a collagen membrane which is useful as a wound-healing biomaterial.
- Subjects
VIETNAM; MOLECULAR weights; COLLAGENASES; GELATIN; COLLAGEN; PATHOGENIC bacteria; SOIL microbiology; CASEINS
- Publication
Bioscience, Biotechnology & Biochemistry, 2020, Vol 84, Issue 11, p2293
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2020.1799748