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- Title
Characterization and gene cloning of l -xylulose reductase involved in l -arabinose catabolism from the pentose-fermenting fungus Rhizomucor pusillus.
- Authors
Yamasaki-Yashiki, Shino; Komeda, Hidenobu; Asano, Yasuhisa; Hoshino, Kazuhiro
- Abstract
l-Xylulose reductase (LXR) catalyzes the reduction ofl-xylulose to xylitol in the fungall-arabinose catabolic pathway. LXR (RpLXR) was purified from the pentose-fermenting zygomycetous fungusRhizomucor pusillusNBRC 4578. The nativeRpLXR is a homotetramer composed of 29 kDa subunits and preferred NADPH as a coenzyme. TheKmvalues were 8.71 mM forl-xylulose and 3.89 mM for dihydroxyacetone. Thelxr3(Rplxr3) gene encodingRpLXR consists of 792 bp and encodes a putative 263 amino acid protein (Mr = 28,341). The amino acid sequence ofRpLXR showed high similarity to 3-oxoacyl-(acyl-carrier-protein) reductase. TheRplxr3gene was expressed inEscherichia coliand the recombinantRpLXR exhibited properties similar to those of nativeRpLXR. Transcription of theRplxr3gene inR. pusillusNBRC 4578 was induced in the presence ofl-arabinose and inhibited in the presence ofd-glucose,d-xylose, andd-mannitol, indicating thatRpLXR is involved in thel-arabinose catabolic pathway.
- Subjects
REDUCTASE genetics; FUNGAL enzyme analysis; ARABINOSE
- Publication
Bioscience, Biotechnology & Biochemistry, 2017, Vol 81, Issue 8, p1612
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2017.1320518