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- Title
Voltammetric Determination of Purine Bases Using a Carbon Electrode Modified With Hybrid Silica Film.
- Authors
Rozhanchuk, Tetiana; Mariia, Velychko; Titov, Maksim; Tananaiko, Oksana
- Abstract
Hybrid silica films containing cation-exchange polyelectrolytes were designed and used to modify a glassy carbon electrode (GCE) for voltammetric determination of purine bases. Hybrid silica-polyelectrolyte films synthesised in the presence of adenine as structure directed component have demonstrated enhanced sorption capacity to purine base. The anodic peaks of adenine and guanine at a hybrid film-modified GCE were observed at +1.55 and +1.1 V, respectively, in phosphate buffer solution at pH 3.5. Oxidation currents of adenine and guanine were proportional to their concentration in the range of 0.02-0.50 mM, with a detection limit of 0.015 mM. The developed method was used to determine adenine in adenosine triphosphate and purine bases in hydrolyzed solutions of DNA and demonstrated good metrological characteristics.
- Subjects
PURINES; CARBON electrodes; SILICA films; GUANINE; OXIDATION; POLYELECTROLYTES; VOLTAMMETRY
- Publication
Electroanalysis, 2013, Vol 25, Issue 9, p2045
- ISSN
1040-0397
- Publication type
Article
- DOI
10.1002/elan.201300081