We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Enrichment of Specifically Labeled Proteins by an Immobilized Host Molecule.
- Authors
Murray, James; Sim, Jaehwan; Oh, Kyunghoon; Sung, Gihyun; Lee, Ara; Shrinidhi, Annadka; Thirunarayanan, Ayyavu; Shetty, Dinesh; Kim, Kimoon
- Abstract
Chemical proteomics relies primarily on click-chemistry-based protein labeling and biotin-streptavidin enrichment, but these techniques have inherent limitations. Enrichment of intracellular proteins using a totally synthetic host-guest complex is described, overcoming the problem associated with the classical approach. We achieve this by affinity-based protein labeling with a target-specific probe molecule conjugated to a high-affinity guest (suberanilohydroxamic acid-ammonium-adamantane; SAHA-Ad) and then enriching the labeled species using a cucurbit[7]uril bead. This method shows high specificity for labeled molecules in a MDA-MB-231 breast cancer cell lysate. Moreover, this method shows promise for labeling proteins in live cells.
- Subjects
PROTEIN affinity labeling; PROTEOMICS; CLICK chemistry; BIOTIN; STREPTAVIDIN; HOST-guest chemistry
- Publication
Angewandte Chemie, 2017, Vol 129, Issue 9, p2435
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201611894