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- Title
Soybean oil content QTL mapping and integrating with meta-analysis method for mining genes.
- Authors
Zhao-ming Qi; Qiong Wu; Xue Han; Ya-nan Sun; Xiang-yu Du; Chun-yan Liu; Hong-wei Jiang; Guo-hua Hu; Qing-shan Chen
- Abstract
Oil content of soybean was a valuable quantitative trait controlled by multiple genes. Eleven QTLs were detected by both CIM and MIM method with the population crossed between Charleston and Dong nong594 in recent 3 years (2007, 2008, 2009). Combining the QTLs collected over the past 20 years, an integrated map of oil-content major QTLs in soybean was established using soymap2, which was published in 2004, as a reference. Using the software BioMercator ver.2.1, QTLs were projected from their own maps onto the reference map. In total, ninety-eight QTLs were integrated into soymap2. A meta-analysis method was used to narrow down the confidence interval, and 20 consensus QTLs and their corresponding markers were obtained. Using a local version of GENSCAN, 10,137 sequences in the consensus QTL intervals were predicted. With BLAST, these predicted genes were compared to the International Protein Index database to mine the related genes. The results offer a basis for gene mining and molecular breeding in soybean.
- Subjects
SOY oil; TRAIT intercorrelations; CROP genetics; SOYBEAN; META-analysis; GENES; PLANT breeding
- Publication
Euphytica, 2011, Vol 179, Issue 3, p499
- ISSN
0014-2336
- Publication type
Article
- DOI
10.1007/s10681-011-0386-1