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- Title
A sampling of methods to study chromosome and genome structure and function.
- Authors
Sullivan, Beth A.
- Abstract
Chromosomes were termed by Waldeyer ([24]), building on Theodor Boveri's description of chromatic elements and Walther Flemming's observations of the longitudinal splitting of threadlike material in the nucleus (i.e., mitosis) (reviewed in Opitz ([20])). The authors present a historical timeline of microscopy-based approaches, from early light microscopy of chromosome banding using synthetic dyes to higher resolution fluorescence microscopy approaches that use designer oligonucleotide Oligopaint probes to identify regions of chromosomes or illuminate entire chromosomes. The article outlines methods such as DRIP-seq and RDIP that globally profile RNA-DNA heteroduplexes and larger RNA-DNA-chromatin protein complexes in the context of gene regulation and 3D genome organization.
- Subjects
CHROMOSOME structure; CHROMOSOME analysis; PLANT cytogenetics; CYTOGENETICS; CHROMOSOME replication; BACTERIAL chromosomes; HISTONES
- Publication
Chromosome Research, 2020, Vol 28, Issue 1, p1
- ISSN
0967-3849
- Publication type
Editorial
- DOI
10.1007/s10577-020-09629-y