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- Title
Identification of rice black-streaked dwarf fijivirus in maize with rough dwarf disease in China.
- Authors
Fang, S.; Yu, J.; Feng, J.; Han, C.; Li, D.; Liu, Y.
- Abstract
Summary. Three virus isolates from maize with rough dwarf in different provinces in China were analyzed at the molecular level. When compared to an isolate from diseased rice plants in Hubei Province, all four isolates had identical genomic RNA electrophoretic profiles, which were composed of ten double-stranded (ds) RNAs. Full-length cDNAs of segment 10 (S10) from each of the four isolates were cloned by RT-PCR and the complete sequences were determined. Analysis of the sequences revealed that each consisted of 1801 nucleotides and contained a single open reading frame (ORF) which potentially encoded a protein with 558 amino acids. Further, the sequences showed more than 97.0% and 98.0% identity atnucleotide and amino acid levels, respectively. In addition, theiridentities to rice black-streaked dwarf virus S10 were significantlyhigher than those to maize rough dwarf virus S10. Based on theseresults, it is suggested that the virus which causes this maize diseasein China is rice black-streaked dwarf virus.
- Subjects
CHINA; CORN diseases; PLANT diseases; RNA; VIRUS diseases of plants; NUCLEOTIDE sequence
- Publication
Archives of Virology, 2001, Vol 146, Issue 1, p167
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s007050170200