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- Title
Tubularized Urethral Replacement Using Tissue-Engineered Peritoneum-Like Tissue in a Rabbit Model.
- Authors
Gu, Gang-Li; Xia, Shu-Jie; Zhang, Jie; Liu, Guo-Hua; Yan, Lei; Xu, Zhong-Hua; Zhu, Ying-Jian
- Abstract
Introduction: This study aims to investigate whether mesothelial cells could function as seed cells to construct tissue-engineered peritoneum-like tissue for urethral reconstruction in a rabbit model. Materials and Methods: Bladder acellular matrices were prepared and trimmed to 1.5 × 1 cm. Nine male rabbits underwent omentum biopsy and autologous mesothelial cells were isolated. After in vitro expansion, the cells were seeded onto the matrices and incubated for 7 days. In 18 rabbits, a pendulous urethral segment 1.5 cm long was totally excised and urethroplasty was performed with tubularized matrices seeded with cells in 9 animals and without cells in 9 as controls. Urethrography was performed at 1, 2 and 6 months postoperatively. Meanwhile, the neourethras were harvested and analyzed grossly and histologically. Results: Histological analysis of the cell-seeded grafts revealed a loose collagen matrix covered with a single layer of mesothelim. Serial urethrography indicated a wide urethral caliber without stricture formation in animals implanted with cell-seeded matrices, while all animals of the control group developed stricture. Histological analysis of the implanted cell-seeded matrices demonstrated a normal urethral architecture by 1 month, composed of multilayers of urothelium surrounded by smooth muscle bundles, which became increasingly organized with time. By 6 months, the neourethra could be hardly distinguished from native urethra both grossly and histologically. Conclusions: Autologous mesothelial cells could be successfully used as seed cells for tubularized urethral reconstruction in male rabbits. Copyright © 2012 S. Karger AG, Basel
- Subjects
URETHROPLASTY; TISSUE engineering; PERITONEUM; TUBES; MESOTHELIUM; URETHRA; LABORATORY rabbits; TRANSPLANTATION of organs, tissues, etc.
- Publication
Urologia Internationalis, 2012, Vol 89, Issue 3, p358
- ISSN
0042-1138
- Publication type
Article
- DOI
10.1159/000339745