We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Synergistic post-antibiotic effect of amikacin and β-lactam antibiotics on Enterococcus faecalis.
- Authors
Isaksson, Barbro; Hanberger, Håkan; Maller, Rolf; Nilsson, Lennart E.; Nilsson, Maud
- Abstract
The in-vitro post-antibiotic effect (PAE) of amikacin alone and in combination with ceftazidime, ceftriaxone and piperacillin was studied for two strains of Enterococcus faecalis using a bioluminescent assay of bacterial ATP. The two strains of E.faecalis were resistant to amikacin, ceftazidime and ceftriaxone but sensitive to piperacillin. The bacterial cultures were incubated with the β-lactam antibiotics for 1 h and concentrations of amikacin between 2–64 mg/l were then added. Thereafter, incubation continued with the combinations for one more hour. After dilution, regrowth was monitored by measuring bacterial ATP every hour. Increasing concentrations of amikacin (2–64 mg/l), ceftazidime (8–32 mg/l) and ceftriaxone (32–128 mg/l) resulted in little or no PAE (0–0·3 h) on these strains. PAEs of 0·5 to 1·6 h resulted from exposure to piperacillin (4–32 mg/l). In combination amikacin and piperacillin increased the PAE to 5·5 h. A synergistic PAE was also seen when the enterococci were exposed to amikacin combined with ceftazidime or ceftriaxone in concentrations close to the MICs of the latter antibiotics.
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 1991, Vol 27, Issue suppl_C, p9
- ISSN
0305-7453
- Publication type
Article